http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Search&db=PubMed&term=%28lipoproteins%20and%20synapse%29%20or%20%28lipoprotein%20and%20synapse%29 Lipoproteins and synapse collection by Neurobiology of Lipids (ISSN 1683-5506) - alerts interested readers about latest peer reviewed scholar publications on neuroscience of lipoproteins en-us http://blogs.law.harvard.edu/tech/rss 1440 http://neurobiologyoflipids.org/http://neurobiologyoflipids.org/images/neurobiologyoflipidslogo250x50.jpg lipoproteinsynapsehttp://feedburner.google.comSubscribe with My Yahoo!Subscribe with NewsGatorSubscribe with My AOLSubscribe with BloglinesSubscribe with NetvibesSubscribe with GoogleSubscribe with PageflakesSubscribe with PlusmoSubscribe with FeedLoungeSubscribe with The Free DictionarySubscribe with Bitty BrowserSubscribe with Live.comSubscribe with Excite MIXSubscribe with Yourminis.comSubscribe with Attensa for OutlookSubscribe with WebwagSubscribe with netomat HubSubscribe with Daily RotationSubscribe with Podcast ReadySubscribe with FlurrySubscribe with ParticlsAdd to Any Feed ReaderThis is Neurobiology of Lipids specialist content summary only. Click on the Journal logo above to visit NoL home page for original content, other collections, and more http://rss.neurobiologyoflipids.org/~r/lipoproteinsynapse/~3/vIW_WRQwJFQ/query.fcgi <table border="0" width="100%"><tr><td align="left"><a href="http://linkinghub.elsevier.com/retrieve/pii/S0304-3940(09)01514-6"><img src="http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif" border="0"/></a> </td><td align="right"><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&amp;cmd=Display&amp;dopt=PubMed_PubMed&amp;from_uid=19932737">Related Articles</a></td></tr></table> <p><b>GFAP reactivity, apolipoprotein E redistribution and cholesterol reduction in human astrocytes treated with alpha-synuclein.</b></p> <p>Neurosci Lett. 2010 Jan 18;469(1):11-4</p> <p>Authors: Koob AO, Paulino AD, Masliah E</p> <p>Alpha-synuclein (alpha-syn) is an abundant neuronal protein expressed at the synapse. In neurodegenerative disease alpha-syn accumulates in the extracellular space. Astrocytes present at neural synapses are thought to contribute to synaptogenesis through cholesterol release and normally exhibit increased glial fibrillary acid protein (GFAP) reactivity and apolipoprotein E (apoE) expression in neurodegenerative disease states. We proposed that extracellular alpha-syn treatment of human astrocytes would impact cholesterol levels and expression of GFAP and apolipoprotein E (apoE). Human astrocytes were treated with alpha-syn at different concentrations and time points to determine the effective membrane permeability of the peptide. After alpha-syn treatment, we analyzed apoE and cholesterol levels in the astrocyte membrane. Lastly, we performed immunocytochemistry for GFAP in control and alpha-syn treated cells. Our results indicate membrane apoE was reduced and redistributed from a nuclear and membranous dominated expression to the cytosol. Cholesterol levels were also reduced in the astrocyte cell membrane. GFAP expression was sharply increased in alpha-syn treated cells indicating that alpha-syn may contribute to reactive gliosis. Our results support the conclusion that astrocytes play a role in pathological mechanisms in synucleinopathies.</p> <p>PMID: 19932737 [PubMed - indexed for MEDLINE]</p><img src="http://feeds.feedburner.com/~r/lipoproteinsynapse/~4/vIW_WRQwJFQ" height="1" width="1"/> Koob AO, Paulino AD, Masliah E Neurosci Lett PubMed:19932737 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=19932737&dopt=Abstract