http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=pubmed&term=%28phospholipid%20synapse%29%20or%20%28phospholipids%20synapse%29 Phospholipids and synapse collection by Neurobiology of Lipids (ISSN 1683-5506) - alerts interested readers about latest peer reviewed scholar publications on neuroscience of phospholipids en-us http://blogs.law.harvard.edu/tech/rss 1440 http://neurobiologyoflipids.org/http://neurobiologyoflipids.org/images/neurobiologyoflipidslogo250x50.jpg 1640990http://www.feedburner.comSubscribe with My Yahoo!Subscribe with NewsGatorSubscribe with My AOLSubscribe with RojoSubscribe with BloglinesSubscribe with NetvibesSubscribe with GoogleSubscribe with PageflakesSubscribe with PlusmoSubscribe with FeedLoungeSubscribe with The Free DictionarySubscribe with Bitty BrowserSubscribe with Live.comSubscribe with Excite MIXSubscribe with Yourminis.comSubscribe with Attensa for OutlookSubscribe with WebwagSubscribe with netomat HubSubscribe with Daily RotationSubscribe with Podcast ReadySubscribe with FlurrySubscribe with ParticlsAdd to Any Feed ReaderSubscribe with fwickiSubscribe with Zune MarketplaceThis is Neurobiology of Lipids specialist content summary only. Click on the Journal logo above to visit NoL home page for original content, other collections, and more http://rss.neurobiologyoflipids.org/~r/phospholipidsynapse/~3/331207681/query.fcgi <table border="0" width="100%"><tr><td align="left"/><td align="right"><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&amp;cmd=Display&amp;dopt=PubMed_PubMed&amp;from_uid=18432988">Related Articles</a></td></tr></table> <p><b>Supported planar bilayers for study of the immunological synapse.</b></p> <p>Curr Protoc Immunol. 2007 Feb;Chapter 18:Unit 18.13</p> <p>Authors: Dustin ML, Starr T, Varma R, Thomas VK</p> <p>Supported planar bilayers have been used in immunology research for over 25 years, including in the initial demonstrations of MHC-peptide complex functional activity and adhesion molecule activity. More recent modifications of the method have been used to measure two-dimensional affinities and to study the formation of the immunological synapse. This unit covers the incorporation of glycolipid-anchored membrane proteins, 6-histidine-tagged soluble proteins, and monobiotinylated soluble proteins into supported planar bilayers. Reagents developed for the MHC-peptide tetramer staining method (UNIT 17.3) can readily be adapted to presentation on planar bilayers. The unique advantage of this approach is that the proteins presented on the surface of the supported bilayer are laterally mobile. This provides a more physiological presentation of cell-surface molecules and supports visualization of protein rearrangement on the bilayer by live cells.</p> <p>PMID: 18432988 [PubMed - indexed for MEDLINE]</p><img src="http://rss.neurobiologyoflipids.org/~r/phospholipidsynapse/~4/331207681" height="1" width="1"/> Dustin ML, Starr T, Varma R, Thomas VK Curr Protoc Immunol PubMed:18432988 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=18432988&dopt=Abstract